L1210/0 cells are maintained in an exponential suspension culture at 37 ℃ in a humidified atmosphere of 5% CO 2 in McCoy's medium 5a (modified), supplemented with 15% calfserum, and Fungizone. L1210/0 cells are incubated in Cisplatin (7 μg/mL) for 2 hr at 37 ℃. To measure growth inhibition, the cells are centrifuged, washed once, resuspended in fresh medium at 30 × 10 3 to 50 × 10 3 cells/mL, and incubated for 3 days. Cell numbers are determined on a Coulter Counter. An aliquot of cells is diluted with an equal volume of % trypan blue. Viability is recorded as the percentage of cells that has excluded trypan blue. Cells incubated with Cisplatin as above are also diluted into % agar and allowed to grow for 2 weeks when colonies are counted.
Francis Bacon, Philosopher and Writer: Bacon has been a traditional favorite of the anti-Stratford camp, and retains a high place on the list of potential candidates. Bacon proponents point toward Bacon's learning, his correspondences and memoirs (most notably, his notebook, Promus ), as well as ciphers and other coincidences. Although Bacon was an undisputed man of letters, his style and expression vary greatly from that of Shakespeare's works. Bacon also produced such a voluminous output of his own, it's hard to conceive of him finding spare time enough to produce the quality output of work attributed to the Bard.